ADVANCED NUTRITIONAL BIOCHEMISTRY II-PRACTICAL

Paper Code: 
24HFN225
Credits: 
04
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Analyze and estimate the micronutrient content in various food samples.
2. Analyze and estimate the total antioxidant activity of food samples
 
Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course

title

24HFN225

 

Advanced Nutritional Biochemistry II-Practical

(Practical)

 

COFN65: Estimate content of the vitamins, minerals antioxidants, flavonoids and antioxidant activity colorimetrically

COFN66: Determine content of vitamin and minerals titrimetricaly and to assess the haemoglobin level in blood.

COFN 67: Compile comprehensive practical

records for estimation of food components,

haemoglobin and cultivate verbal

communication skills to converse

effectively with examiners

COFN68: Contribute effectively in course-specific interaction

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, Informative videos, group discussion

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz, Poster Presentations,

Individual and group projects,

Semester End Examination, discussion, demonstration

 

 

CONTENTS
Titrimetric estimations
• Vitamin C in food samples
• Calcium in food samples
Colorimetric determination of 
• Calcium in food samples
• Iron in food samples
• Inorganic phosphorus in food samples
• Vitamin C in food samples
• Estimation of Vitamin A
• Estimation of hemoglobin by haemoglobinometer
• Estimation of total polyphenols
• Estimation of flavonoids
• Analysis of antioxidant activity by DPPH & FRAP
Essential Readings: 
  1. Sadasivam S and Manickam A (2011). Biochemical methods. New Age International Publisher.
  2. Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications            
  3. William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
  4. Sharma S, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi

 

Academic Year: 
2024-2025
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