NUTRITIONAL BIOCHEMISTRY II-PRACTICAL

Paper Code: 
24DHFN812
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Analyze and estimate the micronutrient content in various food samples.
  2. Analyze and estimate the total antioxidant activity of food samples.
Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course

Title

24DHFN 812

Nutritional Biochemistry II--Practical

(Practical)

CO311:Estimate calorimetrically the content of vitamins, minerals,total polyphenols, flavonoids and antioxidant activity

CO312: Analyze the micronutrient content by titrimetric method and estimate the Haemoglobin level in blood

CO313: Compile comprehensive practical records and culminate verbal communication skills to converse effectively with examiner

CO314: Contribute effectively in course-specific interaction

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, Informative videos, group discussion

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz, Poster Presentations,

Individual and group projects,

Semester End Examination, discussion, demonstration

 

 

Unit I: 
CONTENTS

1.     Titrimetric estimations

●        Vitamin C in food samples

●        Calcium in food samples

2.     Colorimetric determination of

●        Calcium in food samples

●        Iron in food samples

●        Inorganic phosphorus in food samples

●        Vitamin C in food samples

3.     Estimation of Vitamin A

4.     Estimation of hemoglobin by haemoglobinometer

5.     Estimation of total polyphenols

6.     Estimation of flavonoids

7.     Analysis of antioxidant activity by DPPH & FRAP

Essential Readings: 
  • Sadasivam S and Manickam A (2011). Biochemical methods. New Age International Publisher.
  • Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications            
  • William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
  • Sharma S, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
Academic Year: