TECHNIQUES AND INSTRUMENTATION IN NUTRITION RESEARCH

• Electrolytic Dissociation: -

1. Acid, base, salts, buffer, indicator
2. Henderson Hasselbach equation
3. Titratable acidity and pH and its measurement using indicators.

• Physio- chemical principles:

1. Involved in colorimetery
2. Photometry          
3. Fluorometry atomic absorption
4. Flame photometry, their application in the laboratory.

• Chromatography:

1. Principles in absorption
2. Partition and ion exchange chromatography, their application in column paper, TCL and GL chromatography.

• Electrophoresis:

1. Principles, its applications in separation and purification of amino acid and proteins.

• Estimation of Proximate composition:

1. Moisture, fat, carbohydrate, crude protein, fiber, ash.

• Quantitative estimation of vitamins:

vitamin-C and vitamin A/ β carotene

• Quantitative estimation of minerals:

calcium, iron, sodium, potassium and phosphorus

• Microbial assay
  
  1. Different methods of sterilization and disinfections
  2. Preparation of media for various tests.
  3. Standard Plate Count for water and foods
  4. Staining :- Bacterial and Fungal Staining
  5. Water potability tests

• Nutritional assessment, intervention & evaluation 

     a) Nutrition Assessment Methods:- Anthropometry, Dietary, Biochemical, Clinical

    b).Prevalent nutritional deficiency diseases and chronic degenerative diseases         c).Nutrition intervention, use of lesser explored locally available fruits and vegetables, role of antioxidants in preventing nutritional deficiencies and chronic diseases.

    d).Nutrition monitoring and evaluation