Develop an understanding of the principles that underlie biochemical estimations
Develop skills in both qualitative and quantitative estimations
Contents:
Qualitative analysis of monosaccharides, disaccharides and polysaccharides
Qualitative analysis of amino acids and proteins
Estimation of moisture and ash content of foodstuffs
Estimation of acid value of fats and oils
Estimation of vitamin-C by titrimetric method
Estimation of calcium using EDTA by titration
Qualitative testing of food adulterants – metanil yellow in turmeric, arhar dal and yellow sweets; vanaspati in pure ghee; chalk powder and sand in wheat flour; lead chromate in turmeric powder; starch in milk.
Paper chromatography
Essential Readings:
Murray R.K. Granner, D.K., Mayes P.A. and Rodwell V.W. (1993) 23rd Ed. Harper’s Biochemistry, Lange Medical Book
Rama Rao, A.V.SS : A Text Book of Biochemistry L.K. and S. Publishers, Tanuka.
West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
Lehninger A.L., Nelson D.L. and Cox. M.M. (1993) 2nd Ed. Principles of Biochemistry CBS Publishers and Distributors.
Devlin T.M. (1986) 2nd Ed. Text Book of Biochemistry with Clinical Correlation, John Wiley and Sons.
Fruton J and Symond S. General Biochemistry, Asia Publishing House, Bombay.
Indian Standards Institution (1985) ISI Hand Book of Food Analysis Parts I to XI, Manak Bhawan, New Delhi.
Talwar G.P. Text Book of Biochemistry and Human Biology, Prentice Hall of India, New Delhi.
Kahn Conn, EE Stamf P.K. Outlines of Biochemistry Willey Eastern Pvt. Ltd. New Delhi.
Nagar, R and Nair, S. Biochemistry. Rajasthan Hinidi Granth Academy, Jaipur 2001.
Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.