NUTRITIONAL BIOCHEMISTRY (PRACTICAL)

Paper Code: 
CCND-202
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Objectives:

This course will enable the students:

  • Understand of the principles that underlie biochemical estimations
  • Apply the techniques used in qualitative and quantitative analysis 

 

Course outcomes

 

Learning and teaching strategies

Assessment

Strategies

  
 

The students will be able to –

CO 14 : Develop the understanding of good laboratory practices in a biochemistry laboratory.

CO 15: Implement the safety and precautionary measures for working in a laboratory.

CO 16 : Implement the principles and techniques used in the qualitative analysis of carbohydrates and proteins

CO 17 : Apply the principles and techniques used in the estimation of minerals and vitamins

CO 18: Investigate the techniques used to identify the common adulterants present in food items

Approach in teaching:

Demonstration, Discussion

 

Learning activities for the students:

Hands on experience of food product analysis

 

Class test, CA test, Semester end examinations, Quiz, Assignments, Presentation,

  

 

CONTENTS

 

• Qualitative analysis of monosaccharides, disaccharides and polysaccharides

 

• Qualitative analysis of amino acids and proteins

 

• Estimation of moisture and ash content of foodstuffs

 

• Estimation of acid value of fats and oils

 

• Estimation of vitamin-C by titrimetric method

 

• Estimation of calcium using EDTA by titration

 

• Qualitative testing of food adulterants – metanil yellow in turmeric, arhar dal and yellow sweets; vanaspati in pure ghee; chalk powder and sand in wheat flour; lead chromate in turmeric powder; starch in milk.

 

• Paper chromatography 

 

 

                                    

 

 

 

 

Essential Readings: 

TEXT BOOKS:

  1. Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada
  2. Harper, H.A. Review of Physiological Chemistry. Lange medical Library
  3. Rama Rao, A.V.SS : A Text Book of Biochemistry L.K. and S. Publishers, Tanuka.

 

REFERENCE BOOKS

• West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.

• Lehninger A.L., Nelson D.L. and Cox. M.M. (1993) 2nd Ed. Principles of Biochemistry CBS Publishers and Distributors.

• Devlin T.M. (1986) 2nd Ed. Text Book of Biochemistry with Clinical Correlation, John Wiley and Sons.

• Fruton J and Symond S. General Biochemistry, Asia Publishing House, Bombay.

• Nagar, R and Nair, S. Biochemistry. Rajasthan Hinidi Granth Academy, Jaipur 2001.

• Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.

• Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi (1993).

• Stryu L. (1995) Biochemistry Freeman WH & Co.

• Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.

• Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.

• William S, 16th Ed. JAOAC Official Methods of Analysis of the Association of Official Analytical Chemists.

• White, A. Handar, P. Smith E.L. Stelten D.W. (1959) 2nd Ed. Principles of Biochemistry McGraw Hill Book Co.

• Dutt, Debjani R. – How best to plan  and build your home – Pustak Mahal Delhi.

• Sharma Sheel , Experiments and techniques in biochemistry, Galgotia Publishers , Daryaganj- New Delhi 

Academic Year: 
2023-2024
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