NUTRITIONAL BIOCHEMISTRY (PRACTICAL)

Paper Code: 
DHSC 514(A)
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives (COs):

This course will enable the students to –

1. Understand of the principles that underlie biochemical estimations

2. Apply the techniques used in qualitative and quantitative analysis  

Course Outcomes (COs):

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

  
 

The students will be able to –

CO205: Develop the understanding of good laboratory practices in a biochemistry laboratory.

CO206: Apply the principles and techniques used in the estimation of macro and micronutrients

CO207: Apply the principles and techniques used in the estimation acid value of oils & fats.

CO208: Implement the principles and techniques used in paper chromatography

CO209: Examine the presence of common adulterants in food items

Approach in teaching:

Demonstration, Discussion

 

Learning activities for the students:

Hands on experience of food product analysis

 

Class test, CA test, Semester end examinations, Quiz, Assignments, Presentation,

  

 

CONTENTS

  • Qualitative analysis of monosaccharides, disaccharides and polysaccharides
  • Qualitative analysis of amino acids and proteins
  • Estimation of moisture and ash CONTENTS of foodstuffs
  • Estimation of acid value of fats and oils
  • Estimation of vitamin-C by titrimetric method
  • Estimation of calcium using EDTA by titration
  • Qualitative testing of food adulterants – metanil yellow in turmeric, arhar dal and yellow sweets; vanaspati in pure ghee; chalk powder and sand in wheat flour; lead chromate in turmeric powder; starch in milk.
  • Paper chromatography

 

Essential Readings: 
  • Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada
  • Harper, H.A. Review of Physiological Chemistry. Lange medical Library
  • Rama Rao, A.V.SS : A Text Book of Biochemistry L.K. and S. Publishers, Tanuka.

 

 

References: 

•  West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.

•  Lehninger A.L., Nelson D.L. and Cox. M.M. (1993) 2nd Ed. Principles of Biochemistry CBS Publishers and Distributors.

•  Devlin T.M. (1986) 2nd Ed. Text Book of Biochemistry with Clinical Correlation,       John Wiley and Sons.

• Fruton J and Symond S. General Biochemistry, Asia Publishing House, Bombay.

•  Nagar, R and Nair, S. Biochemistry. Rajasthan Hinidi Granth Academy, Jaipur 2001.

•  Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.

•                    Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi (1993).

• Stryu L. (1995) Biochemistry Freeman WH & Co.

•  Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.

•  Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.

•  William S, 16th Ed. JAOAC Official Methods of Analysis of the Association of Official Analytical Chemists.

•  White, A. Handar, P. Smith E.L. Stelten D.W. (1959) 2nd Ed. Principles of Biochemistry McGraw Hill Book Co.

•  Dutt, Debjani R. – How best to plan  and build your home – Pustak Mahal Delhi.

•  Sharma Sheel , Experiments and techniques in biochemistry, Galgotia Publishers , Daryaganj- New Delhi 

REFERENCE JOURNALS:  

·   Journal of Nutritional Biochemistry

·   Journal of Clinical Biochemistry and Nutrition

·    Annual Review of Biochemistry

 

E RESOURCES:

·   Glycolysis - https://www.youtube.com/watch?v=PK8Lf7N5gIA

·   Carbohydrates - https://www.slideshare.net/syed_ismail/carbohydrates-28872311

 ·   Lipids - https://s3.amazonaws.com/ppt-download/chemistryofchemistryoflipids-       150922161707-lva1-app6892.pdf

Academic Year: 
2023-2024
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