Nutritional Biochemistry (Practical)

Paper Code: 
HSC-308
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to:

  • Develop an understanding of the principles that underlie biochemical estimations
  • Develop skills in both qualitative and quantitative estimations
     

 

 

Contents:

  1. Qualitative analysis of carbohydrates
  2. Qualitative analysis of fats and oils
  3. Qualitative analysis of amino acids and proteins
  4. Estimation of moisture and ash content of foodstuffs
  5. Estimation of acid value of fats and oils
  6. Estimation of vitamin-C by titrimetric method
  7. Qualitative testing of food adulterants – metanil yellow in turmeric, arhar dal and yellow sweets; vanaspati in pure ghee; chalk powder and sand in wheat flour; lead chromate in turmeric powder; starch in milk.
  8. Paper chromatography

 

Essential Readings: 
  1. Murray R.K. Granner, D.K., Mayes P.A. and Rodwell V.W. (1993) 23rd Ed. Harper’s Biochemistry, Lange Medical Book
  2. Rama Rao, A.V.SS : A Text Book of Biochemistry L.K. and S. Publishers, Tanuka.
  3.  West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
  4. Lehninger A.L., Nelson D.L. and Cox. M.M. (1993) 2nd Ed. Principles of Biochemistry CBS Publishers and Distributors.
     
References: 
  1. A Practical manual wheeler publishers.
  2. Devlin T.M. (1986) 2nd Ed. Text Book of Biochemistry with Clinical Correlation, John Wiley and Sons.
  3. Fruton J and Symond S. General Biochemistry, Asia Publishing House, Bombay.
  4. Indian Standards Institution (1985) ISI Hand Book of Food Analysis Parts I to XI, Manak Bhawan, New Delhi.
  5. Talwar G.P. Text Book of Biochemistry and Human Biology, Prentice Hall of India, New Delhi.
  6. Kahn Conn, EE Stamf P.K. Outlines of Biochemistry Willey Eastern Pvt. Ltd. New Delhi.
  7. Nagar, R and Nair, S. Biochemistry. Rajasthan Hinidi Granth Academy, Jaipur 2001.
  8. Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.
  9. Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi (1993).
  10. Stryu L. (1995) Biochemistry Freeman WH & Co.
  11. Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
  12. Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
  13. William S, 16th Ed. JAOAC Official Methods of Analysis of the Association of Official Analytical Chemists.
  14. White, A. Handar, P. Smith E.L. Stelten D.W. (1959) 2nd Ed. Principles of Biochemistry McGraw Hill Book Co.
  15. Dutt, Debjani R. – How best to plan  and build your home – Pustak Mahal Delhi.
  16. Sharma Sheel , Experiments and techniques in biochemistry, Galgotia Publishers , Daryaganj- New delhi
     
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