NUTRITIONAL BIOCHEMISTRY I-PRACTICAL

Paper Code: 
24DHFN712
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Understand the basic principles of practical biochemistry
  2. Understand the biochemical methods and approaches used in nutritional biochemistry

 

Course Outcomes: 

Course

Course outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Course Code

Course title

24DHFN 712

 

Nutritional Biochemistry I-Practical

 (Practical)

CO209: Analyze the quality and quantity of carbohydrates, proteins and fats

CO210: Determine the quality of fats & oils and become competent in TLC technique

CO211:Compile comprehensive practical record forqualitative and quantitative analysis of macronutrients and cultivate verbal communication skills to converse effectively with examiners.

CO212:Contribute effectively in

course-specific interaction

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, demonstration

 

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz,

Power Point Presentations, Individual and group projects, Semester End Examination, demonstration

 

 

 

 

Unit I: 
CONTENTS
  • Basic knowledge and working principles of
  •       Spectrophotometry and colorimetry
  •       Chromatography: Paper, TLC, Column
  •       pH
  • Basic knowledge of normality, molatity, percent solutions and dilutions
  • Preparation of buffers and measurement of their pH with indicators and pH meter.
  • Colours reactions for carbohydrates
  • Glucose determination in food and blood by Nelson Somogyi Method.
  • Determination of protein content by Biuret Method.
  • Separation of Amino acids by TLC
  • Estimation of cholesterol using Zlatkis Method.
  • Estimation of
  •       Saponification Number
  •       Free Fatty Acids
  •       Iodine Value
Essential Readings: 
  • Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN       Publications            
  • William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official          Analytical Chemists
  • Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
References: 
  • Journal of Nutritional Biochemistry
  • Journal of Clinical Biochemistry and Nutrition
  • Annual Review of Biochemistry
Academic Year: 
2024-2025
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