NUTRITIONAL BIOCHEMISTRY I-PRACTICAL

Paper Code: 
24CFSN702
Credits: 
2
Contact Hours: 
4.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Understand the basic principles of practical biochemistry
  2. Understand the biochemical methods and approaches used in nutritional biochemistry

 

Course Outcomes: 

Course

Course outcomes

(at course level)

Learning and teaching strategies

Assessment 

Strategies

Course Code

Course title

24CFSN702

Nutritional Biochemistry I- Practical

 (Practical)

CO87: Analyse the quality and quantity of carbohydrates, proteins and fats

CO88: Determine the quality of fats & oils and become competent in TLC technique 

CO89: Compile comprehensive practical record for qualitative and quantitative analysis of macronutrients and cultivate verbal communication skills to converse effectively with examiners.

CO90: Contribute effectively in course-specific interaction

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, demonstration

 

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz, 

Power Point Presentations, Individual and group projects, Semester End Examination, demonstration

 
 

 

12.00
Unit I: 
CONTENTS
  • Basic knowledge and working principles of
  • Spectrophotometry and colorimetry
  • Chromatography: Paper, TLC, Column
  • pH 
  • Basic knowledge of normality, molality, percent solutions and dilutions
  • Preparation of buffers and measurement of their pH with indicators and pH meter.
  • Colours reactions for carbohydrates
  • Glucose determination in food and blood by Nelson Somogyi Method.
  • Determination of protein content by Biuret Method.
  • Separation of Amino acids by TLC
  • Estimation of cholesterol using Zlatkis Method.
    • Estimation of 
    • Saponification Number
    • Free Fatty Acids
    • Iodine Value

 

Essential Readings: 
  • Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications             
  • William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official        Analytical Chemists
  • Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
  • Rastogi S.C. (1993). Biochemistry New Delhi, Tata McGraw Hill Publishing Co. Ltd. Definition, classification of lipids. Simple, Compound and derived
  • Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
  • Stryer L. (1995) Biochemistry Freeman WH & Co.
  • Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
  • Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.

 

 

References: 
  1. Berg, Jeremy Mark, Tymoczko, John L and Stryer. (2002). Biochemistry 5th ed. New York. W.H. Freeman and Co.
  2. Brody Tom. (2004). Nutritional Biochemistry 2nd ed. New Delhi. Elsevier/Reed. Elsevier. India Pvt. Ltd.
  3. Chatterjee, M.N. Shinde and Rana. (2005). Textbook of Medical Biochemistry, 6th ed. New Delhi, Jaypee Brothers. Medical Publisher.
  4. Dandekar Sucheta P. (2002). Medical Biochemistry (Prep Manual for U.G.) 2nd ed. New Delhi B-1 Churchill Livingstone Pvt. Ltd.

 

E Resource:

  1. https://www.slideshare.net/YESANNA/mechanism-of-action-of-hormones-46154204
  2. https://slideplayer.com/slide/5773843/ - Antioxidants
  3. https://www.slideshare.net/YESANNA/detoxification-45599016
  4. https://biochem.zsmu.zp.ua/wp-content/uploads/2017/04/biochemistry-of-vi...

 

Academic Year: 
2024-2025
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