Coming soon
NUTRITIONAL BIOCHEMISTRY
This course will enable the students:
· Understand of the principles that underlie biochemical estimations
· Apply the techniques used in qualitative and quantitative analysis
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Course outcomes
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Learning and teaching strategies |
Assessment Strategies |
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The students will be able to – CO 14 : Develop the understanding of good laboratory practices in a biochemistry laboratory. CO 15: Implement the safety and precautionary measures for working in a laboratory. CO 16 : Implement the principles and techniques used in the qualitative analysis of carbohydrates and proteins CO 17 : Apply the principles and techniques used in the estimation of minerals and vitamins CO 18: Investigate the techniques used to identify the common adulterants present in food items |
Approach in teaching: Demonstration, Discussion
Learning activities for the students: Hands on experience of food product analysis
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Class test, CA test, Semester end examinations, Quiz, Assignments, Presentation, |
CONTENTS
• Qualitative analysis of monosaccharides, disaccharides and polysaccharides
• Qualitative analysis of amino acids and proteins
• Estimation of moisture and ash content of foodstuffs
• Estimation of acid value of fats and oils
• Estimation of vitamin-C by titrimetric method
• Estimation of calcium using EDTA by titration
• Qualitative testing of food adulterants – metanil yellow in turmeric, arhar dal and yellow sweets; vanaspati in pure ghee; chalk powder and sand in wheat flour; lead chromate in turmeric powder; starch in milk.
• Paper chromatography
1. Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada
2. Harper, H.A. Review of Physiological Chemistry. Lange medical Library
3. Rama Rao, A.V.SS : A Text Book of Biochemistry L.K. and S. Publishers, Tanuka.
REFERENCE BOOKS
• West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
• Lehninger A.L., Nelson D.L. and Cox. M.M. (1993) 2nd Ed. Principles of Biochemistry CBS Publishers and Distributors.
• Devlin T.M. (1986) 2nd Ed. Text Book of Biochemistry with Clinical Correlation, John Wiley and Sons.
• Fruton J and Symond S. General Biochemistry, Asia Publishing House, Bombay.
• Nagar, R and Nair, S. Biochemistry. Rajasthan Hinidi Granth Academy, Jaipur 2001.
• Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.
• Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi (1993).
• Stryu L. (1995) Biochemistry Freeman WH & Co.
• Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
• Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
• William S, 16th Ed. JAOAC Official Methods of Analysis of the Association of Official Analytical Chemists.
• White, A. Handar, P. Smith E.L. Stelten D.W. (1959) 2nd Ed. Principles of Biochemistry McGraw Hill Book Co.
• Dutt, Debjani R. – How best to plan and build your home – Pustak Mahal Delhi.
• Sharma Sheel , Experiments and techniques in biochemistry, Galgotia Publishers , Daryaganj- New Delhi
REFERENCE JOURNALS:
1. Journal of Nutritional Biochemistry
2. Journal of Clinical Biochemistry and Nutrition
3. Annual Review of Biochemistry
E RESOURCES:
1. Glycolysis - https://www.youtube.com/watch?v=PK8Lf7N5gIA
2. Carbohydrates - https://www.slideshare.net/syed_ismail/carbohydrates-28872311
