Advanced Nutritional Biochemistry II (Practical)

Paper Code: 
HFN 225
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  • Develop understanding of the molecular principles that underlie biochemical reactions and processes.
  • An understanding of biochemical methods and approaches used in nutritional biochemistry.
  • A foundation of knowledge, understanding and skills required for further study and research.
     

 

Contents:

  1. Titrimetric estimations
  • Vitamin C in food samples
  • Calcium in food samples
  1. Colorimetric determination of
  • Calcium in food samples
  • Iron in food samples
  • Inorganic phosphorus in food samples
  • Vitamin C in food samples
  1. Estimation of beta carotene by column chromatography
  2. Estimation of hemoglobin
  3. Estimation of Vitamin E
  4. Estimation of total polyphoenol
  5. Estimation of flavanoids
Essential Readings: 
  1. Rao, K.R. (1986) Textbook of Biochemistry, III Edition. Prentice Hall of India Pvt. Ltd., New Delhi.
  2. Murray, R.K. Granner, P.A. Mayes, V.W. Rodwell (1988) Harper’s Biochemistry. 21st Edition. Prentice Hall of Australia Pvt. Ltd.
  3. Lehninger, A.L. (1987) Principles of Bio-chemistry – CBS Publishers and Distributors
  4. West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
  5. Robinson C.H., Lawler M.R. ‘Normal and Therapeutic Nutrition’. MacMillan Publishing Company. 17th Edition. 1986.
  6. Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications             
  7. William S, (Latest Edition) AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
  8. Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
     
References: 
  1. Harper, H.A. Review of Physiological Chemistry. Lange medical Library
  2. White, A., Handler, P. and Smith, E.L. Principles of Biochemistry. W. B. Saunders Book Co. Philadelphia.
  3. Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
  4. Stryer L. (1995) Biochemistry Freeman WH & Co.
  5. Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
  6. Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
  7. William S, 16th Ed. JAOAC Official Methods of Analysis of the Association of Official Analytical Chemists
  8. Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi (1993).
  9. Indian Standards Institution (1985) ISI Hand Book of Food Analysis Parts I to XI, Manak Bhawan, New Delhi
  10. Medical Book Ltd Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.
  11. Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann.
     
Academic Year: 
2018-2019
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