ADVANCED NUTRITIONAL BIOCHEMISTRY II (PRACTICAL)

Paper Code: 
HFN 225
Credits: 
04
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
• Develop understanding of the molecular principles that underlie biochemical reactions and processes.
• An understanding of biochemical methods and approaches used in nutritional biochemistry.
• A foundation of knowledge, understanding and skills required for further study and research.
 
Contents:
 
1. .Titrimetric estimations
• Vitamin C in food samples
• Calcium in food samples
2. Colorimetric determination of 
• Calcium in food samples
• Iron in food samples
• Inorganic phosphorus in food samples
• Vitamin C in food samples
3. Estimation of beta carotene by column chromatography
4. Estimation of hemoglobin 
5. Estimation of Vitamin E
6. Estimation of total polyphoenol 
7. Estimation of flavanoids
 
Essential Readings: 
1. Sadasivam S and Manickam A (2011). Biochemical methods. New Age International Publisher. 
2. Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications             
3. William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
4. Sharma S, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
 
 
Academic Year: 
2015-2016
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