ADVANCED NUTRITIONAL BIOCHEMISTRY -I (THEORY)

Paper Code: 
HFN 121
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

1.    Understand the chemistry of macronutrients.

2.    Understand the mechanisms adopted by the human body for regulation of metabolic      pathways.

3.    Understand the interrelationships between various metabolic pathways.

Course outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

  
 

The students will be able to –

COFN 1:Demonstrate the chemistry of macro and micronutrients

COFN 2: Explain the major pathways for metabolism of nutrients and key mechanisms regulating these pathways.

COFN 3: Illustrate the enzyme action and activity, and their diagnostic importance.

COFN 4:Assess the essential functions of nutrients in human cells and tissues. Pathologies associated with common metabolic disorders and defects.

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, Informative videos, group discussion

 

Learning activities for the students:

Self learning assignments, Effective questions, presentations, Field trips

 

 

Quiz, Poster Presentations,

Power Point Presentations, Individual and group projects,

Open Book Test, Semester End Examination, discussion, demonstration

 

 

 

  

 

12.00
Unit I: 
Introduction to biomolecules

·         Types of chemical bonds, significance of asymmetric C atom

·         Carbohydrates Chemistry

·         Classification, structure and functions of carbohydrates

·         Properties of carbohydrates- Physical and chemical

·         Carbohydrate Metabolism Reactions of EMP, TCA (with structures), HMP,

·         Gluconeogenesis (no structures), Glycogen metabolism (no structures),

·         Homeostasis of blood glucose

 

12.00
Unit II: 
Protein Chemistry

·         Classification, structure and functions of amino acids and protein

·         Properties of Protein- Physical and Chemical

·         Plasma proteins – Nature, properties and functions

·         Protein metabolism

·         General reactions of amino acids- transamination, deamination and decarboxylation

·         Detoxification of NH3

  • Krebs-Hensleit cycle, Creatinine and creatine synthesis
12.00
Unit III: 
Lipid Chemistry

·         Classification, structures and functions of lipids

·         Properties of Lipids –Physical and Chemical

·         Structure and functions of Cholesterol

·         Lipid Metabolism

·         Knoop’s β-oxidation of even C fatty acid (no structures), ketone body formation and       utilization

·         Fatty acid biosynthesis of Palmitic acid (no structure)

·         Metabolism of Cholesterol

·         Lipid storage disorders

12.00
Unit IV: 
Enzyme Chemistry

·         Definition, classification IUB (up to 1 digit), Concept of active site Enzyme specificity,      Units: Katal, IU, Factors affecting enzyme activity

·         Definition of Km and significance

·         Enzyme inhibition: Definition of Holoenzyme, Coenzyme, cofactor, Allosteric

·         site, active site, prosthetic group, isoenzyme

·         Mechanism of enzyme action

  • Diagnostic importance of enzymes 
12.00
Unit V: 
Biological Oxidation

·         Electron transport chain; O-R potential; oxidative phosphorylation; enzymes of     biological oxidation; inhibition of respiratory chain and oxidative phosphorylation.

·         Acid-Base balance, Buffers: definition, types of buffers, Role of lungs, kidneys and          haemoglobin in Acid-Base balance Chloride shift

·         Regulation of acid base balance

·         Disorders of Acid-Base imbalance-acidosis & alkalosis –symptoms, causes, diagnosis,      treatment & complications

Essential Readings: 
  • ·         Rao, K.R. (1986) Textbook of Biochemistry, III Edition. Prentice Hall of India Pvt Ltd.,   New Delhi.
  • Murray, R.K. Granner, P.A. Mayes, V.W. Rodwell (1988) Harper’s Biochemistry. 21st        Edition. Prentice Hall of Australia Pvt. Ltd.
  • Lehninger, A.L. (1987) Principles of Bio-chemistry – CBS Publishers and Distributors
  • West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of       Biochemistry, Amerind Publishing Co. Pvt. Ltd.
  • Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada.

 

 

References: 

SUGGESTED READINGS:

  • White, A., Handler, P. and Smith, E.L. Principles of Biochemistry. W. B. Saunders Book Co. Philadelphia.
  • Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
  • Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.
  • Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles Biochemistry, Macmillan Worth Publishers.
  • Devlin, T.M.d (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, WileLissInc
  •  Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.
  •  Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons

 

e RESOURCE:

 

Journal 

  • ·         Journal of Nutritional Biochemistry
  • ·         Journal of Clinical Biochemistry and Nutrition
  • ·         Annual Review of Biochemistry
Academic Year: 
2022-2023
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