Advanced Nutritional Biochemistry-I (Theory)

Paper Code: 
HFN -121
Credits: 
03
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to :

  • Augment the biochemistry knowledge acquired at the undergraduate level.
  • Familiarize the students with the chemistry of macronutrients.
  • Understand the mechanisms adopted by the human body for regulation of metabolic pathways.
  • Get an insight into interrelationships between various metabolic pathways.

 

9.00
Unit I: 
Introduction to Biomolecules

Types of chemical bonds, significance of asymmetric C atom

Carbohydrates Chemistry 

  • Classification, structure and functions of carbohydrates
  • Properties of carbohydrates- Physical and chemical

Carbohydrate Metabolism Reactions of EMP, TCA (with structures), HMP,

  • Gluconeogenesis (no structures), Glycogen metabolism (no structures),
  • Homeostasis of blood glucose

 

9.00
Unit II: 
Protein Chemistry

Protein Chemistry

  • Classification, structure and functions of amino acids and protein
  • Properties of Protein- Physical and Chemical
  • Plasma proteins – Nature, properties and functions

Protein metabolism

  • General reactions of amino acids- transamination, deamination and decarboxylation
  • Detoxification of NH3
  • Krebs-Hensleit cycle, Creatinine and creatine synthesis
9.00
Unit III: 
Lipid Chemistry

Lipid Chemistry

  • Classification, structures and functions of lipids
  • Properties of Lipids –Physical and Chemical
  • Structure and functions of Cholesterol

Lipid Metabolism

  • Knoop’s β-oxidation of even C fatty acid (no structures), ketone body formation
  • and utilization
  • Fatty acid biosynthesis of Palmitic acid (no structure)
  • Metabolism of Cholesterol
  • Lipid storage disorders
9.00
Unit IV: 
Enzyme Chemistry

Enzyme Chemistry

  • Definition, classification IUB (up to 1 digit), Concept of active site, enzyme specificity, turnover number
  • Units: Katal, IU
  • Factors affecting enzyme activity
  • Definition of Km and significance
  • Enzyme inhibition: Definition of Holoenzyme, Coenzyme, cofactor, Allosteric site, active site, prosthetic group, isoenzyme

 

9.00
Unit V: 
Biological Oxidation

Electron transport chain; O-R potential; oxidative phosphorylation; enzymes of biological oxidation; inhibition of respiratory chain and oxidative phosphorylation.

Acid-Base balance, Buffers: definition, types of buffers, Role of lungs, kidneys and haemoglobin in Acid-Base balance Chloride shift

Disorders of Acid-Base imbalance

 

Essential Readings: 
  1. Rao, K.R. (1986) Textbook of Biochemistry, III Edition. Prentice Hall of India Pvt. Ltd., New Delhi.
  2. Murray, R.K. Granner, P.A. Mayes, V.W. Rodwell (1988) Harper’s Biochemistry. 21st Edition. Prentice Hall of Australia Pvt. Ltd.
  3. Lehninger, A.L. (1987) Principles of Bio-chemistry – CBS Publishers and Distributors
  4. West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
  5. Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada.

 

 

References: 
  1. White, A., Handler, P. and Smith, E.L. Principles of Biochemistry. W. B. Saunders Book Co. Philadelphia.
  2. Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
  3. Stryer L. (1995) Biochemistry Freeman WH & Co.
  4. Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
  5. Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
  6. Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.
  7.  Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles Biochemistry, Macmillan Worth Publishers.
  8. Devlin, T.M. (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, Wile Liss Inc
  9. Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.
  10. Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons
  11. Voet, D. Voet, J.G. and Pratt, C.W. (1999). Fundamentals of Biochemistry.
  12. Tietz, N.W. (1976) Fundamentals of Clinical Chemistry. WB Saunders Co.
  13. King, E.J. and Wootton, I.D.P. (1956). 3rd ed. Micro-Analysis in Medical        Biochemistry.  and A Churchill Ltd.
  14. Plummer, D.T. (1987). 3rd ed. An Introduction to Practical Biochemistry.    McGraw-Hill Book Co.

 

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