ADVANCED NUTRITIONAL BIOCHEMISTRY I-PRACTICAL (Practical)

Paper Code: 
25HFN125
Credits: 
04
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Understand the basic principles of practical biochemistry
  2. Understand the biochemical methods and approaches used in nutritional biochemistry

 

Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

 

 

 

 

 

 

 

 

25HFN

125

 

 

 

 

 

 

Advanced Nutritional Biochemistry- Practical (Practical)

COFN25: Analyze the quality and quantity of carbohydrates, proteins and fats

COFN26: Determine the quality of fats & oils and become competent in TLC technique

COFN27: Compile comprehensive practical record for analysis of food quality and nutritive components and cultivate verbal communication skills to converse effectively with examiner

COFN28:           Contribute effectively in

course-specific interaction

Approach in teaching: Interactive Lectures, Discussion, Power Point Presentations, demonstration

Learning activities for the students: Self-learning assignments, Effective questions, presentations, Field trips

Quiz, Power Point

Presentations, Individual and group projects, Semester End Examination, demonstration
   
   
   

 

60.00
Unit I: 
CONTENTS
  • Basic knowledge of normality, molality, percent solutions and dilutions
  • Basic knowledge and working principles of –
    • Spectrophotometer
    • Colorimeter
    • pH meter
    • Chromatography: Paper, TLC, Column
    • Electrophoresis  
  • Preparation of buffers and measurement of their pH
  • Qualitative tests for carbohydrates
  • Separation of Amino acids by TLC
  • Glucose determination in food and blood by Nelson Somogyi Method.
  • Determination of protein content by Biuret Method.
  • Estimation of cholesterol using Zlatkis Method.
  • Estimation of Saponification Number, Acid Value, Iodine Value of fats & oils

 

 

 

Essential Readings: 
  1. Raghuramulu, Nair, and Kalyansundaram (2003) A Manual of Laboratory Techniques, NIN Publications            
  2. William S, (2019) 16th Ed. AOAC Official Methods of Analysis of the Association of Official  Analytical Chemists
  3. Sharma Sheel, (2007), Experiments & Techniques in Biochemistry, Galgotia Publications Pvt Ltd
  4. Rastogi S.C. (1993). Biochemistry New Delhi, Tata McGraw Hill Publishing Co. Ltd. Definition, classification of lipids. Simple, Compound and derived
  5. Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
  6. Stryer L. (1995) Biochemistry Freeman WH & Co.
  7. Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
  8. Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.

 

References: 
  1. Berg, Jeremy Mark, Tymoczko, John L and Stryer. (2002). Biochemistry 5th ed. New York. W.H. Freeman and Co.
  2. Brody Tom. (2004). Nutritional Biochemistry 2nd ed. New Delhi. Elsevier/Reed. Elsevier. India Pvt. Ltd.
  3. Chatterjee, M.N. Shinde and Rana. (2005). Textbook of Medical Biochemistry, 6th ed. New Delhi, Jaypee Brothers. Medical Publisher.
  4. Dandekar Sucheta P. (2002). Medical Biochemistry (Prep Manual for U.G.) 2nd ed. New Delhi B-1 Churchill Livingstone Pvt. Ltd.

 

E Resource:

  1. https://www.slideshare.net/YESANNA/mechanism-of-action-of-hormones-46154204
  2. https://slideplayer.com/slide/5773843/ - Antioxidants
  3. https://www.slideshare.net/YESANNA/detoxification-45599016
  4. https://biochem.zsmu.zp.ua/wp-content/uploads/2017/04/biochemistry-of-vi...

 

 

 

Academic Year: 
2025-2026
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