ADVANCED NUTRITIONAL BIOCHEMISTRY I-PRACTICAL

Paper Code: 
24HFN125
Credits: 
04
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Understand the basic principles of practical biochemistry
  2. Understand the biochemical methods and approaches used in nutritional biochemistry

 

 

Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and teaching strategies

Assessment

Strategies

Course Code

Course title

 

24HFN 125

Advanced Nutritional Biochemistry-Practical

 (Practical)

COFN25: Analyze the quality and quantity of carbohydrates, proteins and fats

COFN26: Determine the quality of fats & oils and become competent in TLC technique

COFN27: Compile comprehensive practical record for analysis of food quality and nutritive components and cultivate verbal communication skills to converse effectively with examiner

COFN28: Contribute effectively in

course-specific interaction

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, demonstration

 

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz,

Power Point Presentations, Individual and group projects, Semester End Examination, demonstration

 

 

 
 

 

Unit I: 
CONTENTS
  • Basic knowledge and working principles of
  • Spectrophotometry and colorimetry
  • Chromatography: Paper, TLC, Column
  • pH
  • Basic knowledge of normality, molatity, percent solutions and dilutions
  • Preparation of buffers and measurement of their pH with indicators and pH meter.
  • Colours reactions for carbohydrates
  • Glucose determination in food and blood by Nelson Somogyi Method.
  • Determination of protein content by Biuret Method.
  • Separation of Amino acids by TLC
  • Estimation of cholesterol using Zlatkis Method..
  • Estimation of
  • Saponification Number
  • Free Fatty Acids
  • Iodine Value

 

Essential Readings: 

 

  1. Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN   Publications            
  2. William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official      Analytical Chemists
  3. Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi

 

Academic Year: 
2024-2025
  • Printer-friendly version
  • PDF version