ADVANCED NUTRITIONAL BIOCHEMISTRY-I (PRACTICAL)

Paper Code: 
HFN 125
Credits: 
03
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to :
• Understand the basic principles of practical biochemistry
• Understand the biochemical methods and approaches used in nutritional biochemistry
 
Contents:
1. Basic knowledge and working principles of
• Spectrophotometry and colorimetry
• Chromatography: Paper, TLC, Column
• pH 
2. Basic knowledge of normality, molatity, percent solutions and dilutions
3. Preparation of buffers and measurement of their pH with indicators and pH meter.
4. Colours reactions for carbohydrates
5. Glucose determination in food and blood by Nelson Somogyi Method.
6. Determination of protein content by Biuret Method.
7. Separation of Amino acids by TLC
8. Estimation of cholesterol using Zlatkis Method..
9. Estimation of 
• Saponification Number
• Free Fatty Acids
• Iodine Value
 
Essential Readings: 
1. Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications             
2. William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
3. Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
 
Academic Year: 
2015-2016
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