Contents:
1. Basic knowledge and working principles of
• Spectrophotometry and colorimetry
• Chromatography: Paper, TLC, Column
• pH
2. Basic knowledge of normality, molatity, percent solutions and dilutions
3. Preparation of buffers and measurement of their pH with indicators and pH meter.
4. Colours reactions for carbohydrates
5. Glucose determination in food and blood by Nelson Somogyi Method.
6. Determination of protein content by Biuret Method.
7. Separation of Amino acids by TLC
8. Estimation of cholesterol using Zlatkis Method..
9. Estimation of
• Saponification Number
• Free Fatty Acids
• Iodine Value