ADVANCE NUTRITIONAL BIOCHEMISTRY-I (PRACTICAL)

Paper Code: 
HFN 125
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course Objectives: 
This course will enable the students to –
1. Understand the basic principles of practical biochemistry
2. Understand the biochemical methods and approaches used in nutritional biochemistry
 
Course Outcomes (COs): 

Course outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

  
 

The students will be able to –

 

COFN16:Competent in handing various chromatographic techniques and apply them in isolating and characterizing different biological molecules.

COFN 17:Calculate the concentration and presence of biomolecules like carbohydrates, proteins, lipids, etc. in known and unknown samples.

COFN 18:Determine saponification value and iodine value of oil and different types of fats.

 

 

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, demonstration

 

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz,

Power Point Presentations, Individual and group projects, Semester End Examination, demonstration

 

 

 

  
 

 

CONTENTS
· Basic knowledge and working principles of
· Spectrophotometry and colorimetry
· Chromatography: Paper, TLC, Column
· pH 
· Basic knowledge of normality, molatity, percent solutions and dilutions
· Preparation of buffers and measurement of their pH with indicators and pH meter.
· Colours reactions for carbohydrates
· Glucose determination in food and blood by Nelson Somogyi Method.
· Determination of protein content by Biuret Method.
· Separation of Amino acids by TLC
· Estimation of cholesterol using Zlatkis Method..
· Estimation of 
· Saponification Number
· Free Fatty Acids
· Iodine Value
 
Essential Readings: 
ESSENTIAL READINGS:
· Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications             
· William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
· Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
 
Journals:  
· Journal of Nutritional Biochemistry
· Journal of Clinical Biochemistry and Nutrition
· Annual Review of Biochemistry
 
Academic Year: 
2022-2023
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