NUTRITIONAL BIOCHEMISTRY (PRACTICAL)

Paper Code: 
DHSC 514 (A)
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Understand of the principles that underlie biochemical estimations
  2. Apply the techniques used in qualitative and quantitative analysis 

Course outcomes

 

Learning and teaching strategies

Assessment

Strategies

  
 

The students will be able to –

CO205: Develop the understanding of good laboratory practices in a biochemistry laboratory.

CO206: Implement the safety and precautionary measures for working in a laboratory.

CO207: Apply the principles and techniques used in the estimation of minerals and vitamins

CO208: Implement the principles and techniques used in the estimation of phytochemicals

CO209: Examine the Haemoglobin level by using Haemoglobinometer

Approach in teaching:

Demonstration, Discussion

 

Learning activities for the students:

Hands on experience of food product analysis

 

Class test, CA test, Semester end examinations, Quiz, Assignments, Presentation,

  

 

CONTENTS
 
• Qualitative analysis of monosaccharides, disaccharides and polysaccharides
 
• Qualitative analysis of amino acids and proteins
 
• Estimation of moisture and ash CONTENTS of foodstuffs
 
• Estimation of acid value of fats and oils
 
• Estimation of vitamin-C by titrimetric method
 
• Estimation of calcium using EDTA by titration
 
• Qualitative testing of food adulterants – metanil yellow in turmeric, arhar dal and yellow sweets; vanaspati in pure ghee; chalk powder and sand in wheat flour; lead chromate in turmeric powder; starch in milk.
 
• Paper chromatography 
 
Essential Readings: 
• Murray R.K. Granner, D.K., Mayes P.A. and Rodwell V.W. (1993) 23rd Ed. Harper’s Biochemistry, Lange Medical Book
 
• Rama Rao, A.V.SS : A Text Book of Biochemistry L.K. and S. Publishers, Tanuka.
 
• West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
 
• Lehninger A.L., Nelson D.L. and Cox. M.M. (1993) 2nd Ed. Principles of Biochemistry CBS Publishers and Distributors.
 
• Devlin T.M. (1986) 2nd Ed. Text Book of Biochemistry with Clinical Correlation, John Wiley and Sons.
• Fruton J and Symond S. General Biochemistry, Asia Publishing House, Bombay.
• Indian Standards Institution (1985) ISI Hand Book of Food Analysis Parts I to XI, Manak Bhawan, New Delhi.
• Talwar G.P. Text Book of Biochemistry and Human Biology, Prentice Hall of India, New Delhi.
• Kahn Conn, EE Stamf P.K. Outlines of Biochemistry Willey Eastern Pvt. Ltd. New Delhi.
• Nagar, R and Nair, S. Biochemistry. Rajasthan Hinidi Granth Academy, Jaipur 2001.
• Oser B.L. (1965) 14th Ed. Hawk’s Physiological Chemistry McGraw Hill Book Co.
• Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi (1993).
• Stryu L. (1995) Biochemistry Freeman WH & Co.
• Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
• Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
• William S, 16th Ed. JAOAC Official Methods of Analysis of the Association of Official Analytical Chemists.
• White, A. Handar, P. Smith E.L. Stelten D.W. (1959) 2nd Ed. Principles of Biochemistry McGraw Hill Book Co.
• Dutt, Debjani R. – How best to plan  and build your home – Pustak Mahal Delhi.
• Sharma Sheel , Experiments and techniques in biochemistry, Galgotia Publishers , Daryaganj- New delhi 
 
Academic Year: 
2022-2023
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