NUTRITIONAL BIOCHEMISTRY II - PRACTICAL

Paper Code: 
24CFSN802
Credits: 
2
Contact Hours: 
4.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Analyze and estimate the micronutrient content in various food samples.
  2. Analyze and estimate the total antioxidant activity of food samples.

 

 

Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and teaching strategies

Assessment Strategies 

Course Code

Course

Title

24CFSN802

Nutritional Biochemistry II-Practical

(Practical)

CO121: Estimate colorimetrically the content of vitamins, minerals, total polyphenols, flavonoids and antioxidant activity 

CO122: Analyze the micronutrient content by titrimetric method and estimate the Haemoglobin level in blood

CO123:  Compile comprehensive practical records for analysing micronutrients, antioxidant activity and culminate verbal communication skills to converse effectively with examiner

CO124: Contribute effectively in course-specific interaction

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, Informative videos, group discussion

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz, Poster Presentations,

Individual and group projects,

Semester End Examination, discussion, demonstration

 

Unit I: 
CONTENTS
  1. Titrimetric estimations
  • Vitamin C in food samples
  • Calcium in food samples

   2. Colorimetric determination of 

  • Calcium in food samples
  • Iron in food samples
  • Inorganic phosphorus in food samples
  • Vitamin C in food samples
  1. Estimation of Vitamin A
  2. Estimation of hemoglobin by haemoglobinometer
  3. Estimation of total polyphenols
  4. Estimation of flavonoids
  5. Analysis of antioxidant activity by DPPH & FRAP

 

Essential Readings: 
  • Sadasivam S and Manickam A (2011). Biochemical methods. New Age International Publisher.
  • Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications             
  • William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
  • Sharma S, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi

 

 

Academic Year: 
2024-2025
  • Printer-friendly version
  • PDF version