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ADVANCED NUTRITIONAL BIOCHEMISTRY-I (THEORY)
Paper Code:
HFN 121
Credits:
03
Contact Hours:
45.00
Max. Marks:
100.00
Objective:
This course will enable the students to :
• Augment the biochemistry knowledge acquired at the undergraduate level.
• Familiarize the students with the chemistry of macronutrients.
• Understand the mechanisms adopted by the human body for regulation of metabolic pathways.
• Get an insight into interrelationships between various metabolic pathways.
9.00
Unit I:
Introduction to biomolecules
Types of chemical bonds, significance of asymmetric C atom
Carbohydrates Chemistry
Classification, structure and functions of carbohydrates
Properties of carbohydrates- Physical and chemical
Carbohydrate Metabolism Reactions of EMP, TCA (with structures), HMP,
Gluconeogenesis (no structures), Glycogen metabolism (no structures),
Homeostasis of blood glucose
9.00
Unit II:
Protein Chemistry
Classification, structure and functions of amino acids and protein
Properties of Protein- Physical and Chemical
Plasma proteins – Nature, properties and functions
Protein metabolism
General reactions of amino acids- transamination, deamination and decarboxylation
Detoxification of NH3
Krebs-Hensleit cycle, Creatinine and creatine synthesis
9.00
Unit III:
Lipid Chemistry
Classification, structures and functions of lipids
Properties of Lipids –Physical and Chemical
Structure and functions of Cholesterol
Lipid Metabolism
Knoop’s β-oxidation of even C fatty acid (no structures), ketone body formation
and utilization
Fatty acid biosynthesis of Palmitic acid (no structure)
Metabolism of Cholesterol
Lipid storage disorders
9.00
Unit IV:
Enzyme Chemistry
Definition, classification IUB (up to 1 digit), Concept of active site, enzyme specificity, turnover number
Units: Katal, IU
Factors affecting enzyme activity
Definition of Km and significance
Enzyme inhibition: Definition of Holoenzyme, Coenzyme, cofactor, Allosteric
site, active site, prosthetic group, isoenzyme
9.00
Unit V:
Biological Oxidation
Electron transport chain; O-R potential; oxidative phosphorylation; enzymes of biological oxidation; inhibition of respiratory chain and oxidative phosphorylation.
Acid-Base balance, Buffers: definition, types of buffers, Role of lungs, kidneys and haemoglobin in Acid-Base balance Chloride shift
Disorders of Acid-Base imbalance
Essential Readings:
1. Rao, K.R. (1986) Textbook of Biochemistry, III Edition. Prentice Hall of India Pvt. Ltd., New Delhi.
2. Murray, R.K. Granner, P.A. Mayes, V.W. Rodwell (1988) Harper’s Biochemistry. 21st Edition. Prentice Hall of Australia Pvt. Ltd.
3. Lehninger, A.L. (1987) Principles of Bio-chemistry – CBS Publishers and Distributors
4. West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
5. Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada.
References:
1. White, A., Handler, P. and Smith, E.L. Principles of Biochemistry. W. B. Saunders Book Co. Philadelphia.
2. Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
3. Stryer L. (1995) Biochemistry Freeman WH & Co.
4. Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
5. Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
6. Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.
7. Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles Biochemistry, Macmillan Worth Publishers.
8. Devlin, T.M. (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, Wile Liss Inc
9. Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.
10. Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons
11. Voet, D. Voet, J.G. and Pratt, C.W. (1999). Fundamentals of Biochemistry.
12. Tietz, N.W. (1976) Fundamentals of Clinical Chemistry. WB Saunders Co.
13. King, E.J. and Wootton, I.D.P. (1956). 3rd ed. Micro-Analysis in Medical Biochemistry. and A Churchill Ltd.
14. Plummer, D.T. (1987). 3rd ed. An Introduction to Practical Biochemistry. McGraw-Hill Book Co.
Academic Year:
