Coming soon
Advanced Nutritional Biochemistry-I (Practical)
This course will enable the students to :
- Understand the basic principles of practical biochemistry
- Understand the biochemical methods and approaches used in nutritional biochemistry
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Course outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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The students will be able to –
COFN16:Competent in handing various chromatographic techniques and apply them in isolating and characterizing different biological molecules. COFN 17:Calculate the concentration and presence of biomolecules like carbohydrates, proteins, lipids, etc. in known and unknown samples. COFN 18:Determine saponification value and iodine value of oil and different types of fats.
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Approach in teaching: Interactive Lectures, Discussion, Power Point Presentations, demonstration
Learning activities for the students: Self-learning assignments, Effective questions, presentations, Field trips |
Quiz, Power Point Presentations, Individual and group projects, Semester End Examination, demonstration
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Contents:
- Basic knowledge and working principles of
- Spectrophotometry and colorimetry
- Chromatography: Paper, TLC, Column
- Electrophoresis: Gel, Paper
- Centrifugation
- pH
- Basic principles of reagent preparation in reference to normality, concentration and dilutions
- Preparation of solutions and reagents for the practical.
- Preparation of buffers and measurement of their pH with indicators and pH meter.
- Colours reactions for carbohydrates
- Estimation of
- Saponification Number
- Iodine Value
- Free Fatty Acid
- Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN Publications
- William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official Analytical Chemists
- Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi
