Advanced Nutritional Biochemistry-I (Practical)

Paper Code: 
HFN 125
Credits: 
04
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

1.     Understand the basic principles of practical biochemistry

2.     Understand the biochemical methods and approaches used in nutritional biochemistry

Course outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

  
 

The students will be able to –

 

COFN16:Competent in handing various chromatographic techniques and apply them in isolating and characterizing different biological molecules.

COFN 17:Calculate the concentration and presence of biomolecules like carbohydrates, proteins, lipids, etc. in known and unknown samples.

COFN 18:Determine saponification value and iodine value of oil and different types of fats.

 

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, demonstration

 

Learning activities for the students:

Self-learning assignments, Effective questions, presentations, Field trips

Quiz,

Power Point Presentations, Individual and group projects, Semester End Examination, demonstration

 

 

 

  
 

 

CONTENTS

·       Basic knowledge and working principles of

·       Spectrophotometry and colorimetry

·       Chromatography: Paper, TLC, Column

·       pH

·       Basic knowledge of normality, molatity, percent solutions and dilutions

·       Preparation of buffers and measurement of their pH with indicators and pH meter.

·       Colours reactions for carbohydrates

·       Glucose determination in food and blood by Nelson Somogyi Method.

·       Determination of protein content by Biuret Method.

·       Separation of Amino acids by TLC

·       Estimation of cholesterol using Zlatkis Method..

·       Estimation of

·       Saponification Number

·       Free Fatty Acids

·       Iodine Value

 

Essential Readings: 

ESSENTIAL READINGS:

·       Raghuramulu, Nair, and Kalyansundaram, A Manual of Laboratory Techniques, NIN        Publications            

·       William S, 16th Ed. AOAC Official Methods of Analysis of the Association of Official       Analytical Chemists

·       Sharma Sheel, Practical Biochemistry. Classic Publishing House, Jaipur-Delhi

 

Journals:

·       Journal of Nutritional Biochemistry

·       Journal of Clinical Biochemistry and Nutrition

·       Annual Review of Biochemistry

 

Academic Year: 
2023-2024
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