ADVANCED NUTRITIONAL BIOCHEMISTRY-I

Paper Code: 
HFN 121
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

 

  1. Understand the chemistry of macronutrients.
  2. Understand the mechanisms adopted by the human body for regulation of metabolic pathways.
  3. Understand the interrelationships between various metabolic pathways.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

 

 

 

 

 

 

 

 

HFN  121

Advanced Nutritional Biochemistry-I

(Theory)

 

 

 

 

 

 

The students will be able to –

 

COFN 1:Demonstrate the chemistry of macro and micronutrients

 

COFN 2: Explain the major pathways for metabolism of nutrients and key mechanisms regulating these pathways.

 

COFN 3: Illustrate the enzyme action and activity, and their diagnostic importance.

 

COFN 4:Assess the essential functions of nutrients in human cells and tissues. Pathologies associated with common metabolic disorders and defects.

Approach in teaching:

Interactive Lectures, Discussion, Power Point Presentations, Informative videos, group discussion

 

Learning activities for the students:

Self learning assignments, Effective questions, presentations, Field trips

 

 

Quiz, Poster Presentations,

Power Point Presentations, Individual and group projects,

Open Book Test, Semester End Examination, discussion, demonstration

 

 

 

 

12.00
Unit I: 
UNIT I

Introduction to biomolecules                                         

  • Types of chemical bonds, significance of asymmetric C atom
  • Carbohydrates Chemistry
    • Classification, structure and functions of carbohydrates
    • Properties of carbohydrates- Physical and chemical
  • Carbohydrate Metabolism Reactions of EMP, TCA (with structures), HMP,
    • Gluconeogenesis (no structures), Glycogen metabolism (no structures),
    • Homeostasis of blood glucose

 

12.00
Unit II: 
UNIT II

Protein Chemistry                                                                                          

  • Classification, structure and functions of amino acids and protein
  • Properties of Protein- Physical and Chemical
  • Plasma proteins – Nature, properties and functions
  • Protein metabolism
    • General reactions of amino acids- transamination, deamination and decarboxylation
    • Detoxification of NH3
    • Krebs-Hensleit cycle, Creatinine and creatine synthesis    

 

12.00
Unit III: 
UNIT III

Lipid Chemistry                                                                                 

  • Classification, structures and functions of lipids
  • Properties of Lipids –Physical and Chemical
  • Structure and functions of Cholesterol
  • Lipid Metabolism
    • Knoop’s β-oxidation of even C fatty acid (no structures), ketone body formation and utilization
    • Fatty acid biosynthesis of Palmitic acid (no structure)
    • Metabolism of Cholesterol
    • Lipid storage disorders

 

12.00
Unit IV: 
UNIT IV

Enzyme Chemistry                                                                                          

  • Definition, classification IUB (up to 1 digit), Concept of active site Enzyme specificity, Units: Katal, IU, Factors affecting enzyme activity
  • Definition of Km and significance
  • Enzyme inhibition: Definition of Holoenzyme, Coenzyme, cofactor, Allosteric
  • site, active site, prosthetic group, isoenzyme
  • Mechanism of enzyme action
  • Diagnostic importance of enzymes

 

12.00
Unit V: 
UNIT V

Biological Oxidation                                                                                      

  • Electron transport chain; O-R potential; oxidative phosphorylation; enzymes of biological oxidation; inhibition of respiratory chain and oxidative phosphorylation.
  • Acid-Base balance, Buffers: definition, types of buffers, Role of lungs, kidneys and haemoglobin in Acid-Base balance Chloride shift
  • Regulation of acid base balance
  • Disorders of Acid-Base imbalance-acidosis & alkalosis –symptoms, causes, diagnosis, treatment & complications

 

Essential Readings: 
  • Rao, K.R. (1986) Textbook of Biochemistry, III Edition. Prentice Hall of India Pvt. Ltd., New Delhi.
  • Murray, R.K. Granner, P.A. Mayes, V.W. Rodwell (1988) Harper’s Biochemistry. 21st Edition. Prentice Hall of Australia Pvt. Ltd.
  • Lehninger, A.L. (1987) Principles of Bio-chemistry – CBS Publishers and Distributors
  • West, E.S. Todd W.R. Mason, H.S. and Van Bruggen J.T (1974) 4th Ed. Text Book of Biochemistry, Amerind Publishing Co. Pvt. Ltd.
  • Satyanarayan U (2002) Biochemistry.Uppala author-publisher interlinks, Vijaywada.

 

 

References: 
  • White, A., Handler, P. and Smith, E.L. Principles of Biochemistry. W. B. Saunders Book Co. Philadelphia.
  • Kahn Conn, E.E. and Stump, P.K. Outlines of Biochemistry. Willey Eastern Pvt. Ltd. New Delhi
  • Stryer L. (1995) Biochemistry Freeman WH & Co.
  • Sundararaj, P and Siddhu A (1995) Qualitative tests and Quantitative Procedures in Biochemsitry.
  • Varley H. Gowenlock, A.H. and Bell, M (1980) 5th Ed. Practical and Clinical Chemistry Vol. I William Heinemann Medical Book Ltd.
  • Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.
  •  Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles Biochemistry, Macmillan Worth Publishers.
  • Devlin, T.M.d (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, WileLissInc
  • Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.
  • Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons
  • Voet, D. Voet, J.G. and Pratt, C.W. (1999). Fundamentals of Biochemistry.
  • Tietz, N.W. (1976) Fundamentals of Clinical Chemistry. WB Saunders Co.
  • King, E.J. and Wootton, I.D.P. (1956). 3rd ed. Micro-Analysis in Medical        Biochemistry.  and A Churchill Ltd.
  • Plummer, D.T. (1987). 3rd ed. An Introduction to Practical Biochemistry.    McGraw-Hill Book Co.

 

 

 

Academic Year: 
2021-2022
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